Cell Cycle Assay Kit (Fluorometric - Green)

DNA quantification Human - Hep G2

Experiment
DNA quantification Human - Hep G2
Product
Cell Cycle Assay Kit (Fluorometric - Green) from Abcam
Manufacturer
Abcam

Protocol tips

Upstream tips
It is not necessary to fix the cells before DNA staining since the Nuclear Green CCS1 is cell- permeable

Publication protocol

Cell-cycle analysis
The cells treated by AFB1 after the miRNA inhibitor and NC transfections were harvested and washed once in phosphate-buffered saline, and fixed in 70% ethanol over night. The DNA content was then examined using the Cell Cycle Analysis Kit of Beyotime (Beijing, China) and analyzed using BD FACS Calibur FlowCytometer. The data were analyzed by ModiFit analysis software.



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Discussion

Discussion

5 years ago

Author: Dorota Ostrowska Poland

About nuclease-free water in DNA quantification

Hi everyone! I have a question about DNA quantification. Do I need to use nuclease-free water for DNA quantification and are there any repercussions if I don’t use any?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA quantification Human - Hep G2 using Cell Cycle Assay Kit (Fluorometric - Green) from Abcam.

Paper title
screened by miRNA profiling negatively regulates / signaling pathway in Aflatoxin B1 induced hepatotoxicity
Full paper
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Manufacturer protocol

Download the product protocol from Abcam for Cell Cycle Assay Kit (Fluorometric - Green) below.

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Videos

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